UPRAVLJANJE PROMOTIVNIM I PRODAJNIM AKTIVNOSTIMA NA PRIMJERU TURISTIČKOG SUBJEKTA : Završni rad

Predmet ovoga rada je bio prikazati načine upravljanja promotivnim i prodajnim aktivnostima kojima se koristi hotel Radisson Blu Resort & Spa Split. U tu svrhu su provedeni intervjui s odgovornim osobama u hotelu, odnosno s direktorom prihoda, menadžerom prodaje i online marketing koordinatorom. Rezultati istraživanja su pokazali da hotel upravlja svojim promotivnim i prodajnim aktivnostima na dobar način. Hotel bilježi zadovoljavajuće prihode, koristi se različitim načinima promocije i prodaje polažući pažnju primarno na web stranicu kao kanal i promocije i prodaje. Osim toga sudjeluje i na raznim sajmovima, koristi prateće načine komunikacije s gostima. Obzirom da postoji nekoliko ciljnih skupina koje su atraktivne hotelu u tržišnom pogledu potrebno je uskladiti sve elemente marketinškog miksa omogućavajući odgovarajuću ponudu svakoj od ciljnih skupina. Slijedom navedenog hotel bi trebao kontinuirano osnaživati promotivne aktivnosti ulažući u nove komunikacijske oblike, te nudeći, gdje je to moguće, 'customizirani' pristup gostu, kao i osmisliti ponudu i za domicilno stanovništvo. Na kraju rada daje se uvid u zaključna razmatranja, ograničenja rada i smjernice za buduća istraživanja.Research subject was to show the ways of managing the promotional and sales activities used by Radisson Blu Resort & Spa Split. For this purpose, the interviews with the responsible employees were conducted, i.e. with revenue manager, sales manager and online marketing coordinator. Research results show that the hotel manages promotional and sales activities very well. Hotel has satisfying revenues, uses different promotional and sales forms paying the attention primarily to web site, which serves both promotional and selling functions. Besides that, hotel attends different trade fairs, uses the variety of supporting communication means. Due to several target markets that are interested to the hotel, it is necessary to coordinate all elements of marketing mix enabling the adequate offer to each of the target segments. In terms of the suggestions, hotel should continue to strengthen promotional activities investing in the new communication forms and to offer, where possible, customized guest approach, as well as to reach the local population. At the end, the paper offers the insights into the concluding remarks, research limitations and future research directions

Characterization of Embryonic Stem (ES) Neuronal Differentiation Combining Atomic Force, Confocal and DIC Microscopy Imaging

The ultimate clinical implementation of embryonic stem cells will require methods and protocols to turn these unspecialized cells into the fully functioning cell types found in a wide variety of tissues and organs. In order to achieve this, it is necessary to clearly understand the signals and cues that direct embryonic stem cell differentiation. This book provides a snapshot of current research on the differentiation of embryonic stem cells to a wide variety of cell types, including neural, cardiac, endothelial, osteogenic, and hepatic cells. In addition, induced pluripotent stem cells and other pluripotent stem cell sources are described. The book will serve as a valuable resource for engineers, scientists, and clinicians as well as students in a wide range of disciplines

Glia in amyotrophic lateral sclerosis and spinal cord injury: common therapeutic targets

The toolkit for repairing damaged neurons in amyotrophic lateral sclerosis (ALS) and spinal cord injury (SCI) is extremely limited. Here, we reviewed the in vitro and in vivo studies and clinical trials on nonneuronal cells in the neurodegenerative processes common to both these conditions. Special focus was directed to microglia and astrocytes, because their activation and proliferation, also known as neuroinflammation, is a key driver of neurodegeneration. Neuroinflammation is a multifaceted process that evolves during the disease course, and can be either beneficial or toxic to neurons. Given the fundamental regulatory functions of glia, pathogenic mechanisms in neuroinflammation represent promising therapeutic targets. We also discussed neuroprotective, immunosuppressive, and stem-cell based approaches applicable to both ALS and SCI

Archaeal aminoacyl-tRNA synthetases interact with the ribosome to recycle tRNAs

Aminoacyl-tRNA synthetases (aaRS) are essential enzymes catalyzing the formation of aminoacyl-tRNAs, the immediate precursors for encoded peptides in ribosomal protein synthesis. Previous studies have suggested a link between tRNA aminoacylation and high-molecular-weight cellular complexes such as the cytoskeleton or ribosomes. However, the structural basis of these interactions and potential mechanistic implications are not well understood. To biochemically characterize these interactions we have used a system of two interacting archaeal aaRSs: an atypical methanogenic-type seryl-tRNA synthetase and an archaeal ArgRS. More specifically, we have shown by thermophoresis and surface plasmon resonance that these two aaRSs bind to the large ribosomal subunit with micromolar affinities. We have identified the L7/L12 stalk and the proteins located near the stalk base as the main sites for aaRS binding. Finally, we have performed a bioinformatics analysis of synonymous codons in the Methanothermobacter thermautotrophicus genome that supports a mechanism in which the deacylated tRNAs may be recharged by aaRSs bound to the ribosome and reused at the next occurrence of a codon encoding the same amino acid. These results suggest a mechanism of tRNA recycling in which aaRSs associate with the L7/L12 stalk region to recapture the tRNAs released from the preceding ribosome in polysome

Colorimetric Assessment of Different Tooth Whitening Procedures

Svrha: Nakana je bila ispitati učinkovitost četiriju različitih postupaka izbjeljivanja te usporediti 30-postotni gel karbamid-peroksid s dva, danas dostupna “in office” postupka izbjeljivanja kod kojih se, osim gela, koristimo i modernim izvorima svjetlosti (Discus Dental, Culver City, CA, SAD i Beyond Technology Corp., Santa Clara, CA, SAD) te klinički još neprimijenjenim femtosekundnim uređajem u koji je ugrađena zelena laserska pumpa (Millennia, Spectra Physics) i femtosekundni laserski oscilator (Tsunami, Spectra Physics). Materijali i metode: U zeleni čaj osam je sati bilo uronjeno 40 pastila hidroksilapatita. Nakon sušenja nasumce su podijeljene u četiri skupine, u svakoj po deset. Svaka skupina tretirana je jednim od četiriju postupaka izbjeljivanja. Boja pastila određena je kolorimetrom u RGB-indeksu prije bojenja u čaju, poslije toga i nakon tretmana određenim postupkom za izbjeljivanje. Za analizu ukupnih kolorimetrijskih vrijednosti koristili smo se i postupcima neparametrijske statistike – Kruskal-Wallisovim i Mann-Whitneyjevim testom za nezavisne uzorke i Wilcoxovim testom za zavisne uzorke. Rezultati: Ukupne kolorimetrijske vrijednosti pastila obrađenih samo gelom bile su 3024,42, onih tretiranih izvorom svjetlosti ZOOM 2 iznosile su 2999,74, pastila tretiranih Beyondom 2944,12 te laserom 2687,22. Najbolji rezultati postignuti su 30-postotnim gelom karbamid-peroksida i ZOOM-om 2, a slijede Beyond i femtosekundni laser, kod kojih je učinak izbjeljivanja bio proporcionalan trajanju tretmana i koncentraciji gela. Zaključak: Iako su se sva četiri postupka izbjeljivanja pokazala uspješnima, konačni rezultat ovisi o trajanju tretmana te vrsti i koncentraciji primijenjenoga gela.Objective: To study the efficiency of four different tooth whitening procedures we examined 30% carbamide peroxide gel alone and compared it with two available in-office whitening methods (Discus Dental, Culver City, CA, USA and Beyond Technology Corp, Santa Clara, CA, USA) and a procedure involving a femtosecond laser which consists of a 6W green laser pump (Millennium, Spectra Physics) and a mode-locked laser (Tsunami, Spectra Physics) which have not been clinically tested so far. Material and Methods: 40 pastilles of hydroxylapatite were used and immersed in green tea for 8 hours. After drying, pastilles were randomly divided into 4 groups, each consisting of 10 pastilles and treated with one of the 4 bleaching procedures. The color of pastilles was determined by the colorimeter in the RGB index prior to immersion into the tea, after the immersion and after the whitening treatment. Non-parametric tests were used for the analysis of colorimetric sum values - Kruskal-Wallis and Mann-Whitney test for independent samples and Wilcoxon test for dependent samples. Results: The colorimetric sum value for the pastilles treated with the gel only was 3024.42, for the pastilles treated with ZOOM 2 it was 2999.74, for the pastilles treated with Beyond it was 2944.12 and for the laser-treated pastilles it was 2687.22. The best results were obtained using the 30% carbamide peroxide gel and ZOOM2, followed by Beyond and the femtosecond laser method, which exhibited a whitening effect proportional to treatment duration and gel concentration. Conclusion: Although all four bleaching methods proved effective, the final result of bleaching depends upon the duration of treatment and the type and concentration of the gel applied

Colorimetric Assessment of Different Tooth Whitening Procedures

Svrha: Nakana je bila ispitati učinkovitost četiriju različitih postupaka izbjeljivanja te usporediti 30-postotni gel karbamid-peroksid s dva, danas dostupna “in office” postupka izbjeljivanja kod kojih se, osim gela, koristimo i modernim izvorima svjetlosti (Discus Dental, Culver City, CA, SAD i Beyond Technology Corp., Santa Clara, CA, SAD) te klinički još neprimijenjenim femtosekundnim uređajem u koji je ugrađena zelena laserska pumpa (Millennia, Spectra Physics) i femtosekundni laserski oscilator (Tsunami, Spectra Physics). Materijali i metode: U zeleni čaj osam je sati bilo uronjeno 40 pastila hidroksilapatita. Nakon sušenja nasumce su podijeljene u četiri skupine, u svakoj po deset. Svaka skupina tretirana je jednim od četiriju postupaka izbjeljivanja. Boja pastila određena je kolorimetrom u RGB-indeksu prije bojenja u čaju, poslije toga i nakon tretmana određenim postupkom za izbjeljivanje. Za analizu ukupnih kolorimetrijskih vrijednosti koristili smo se i postupcima neparametrijske statistike – Kruskal-Wallisovim i Mann-Whitneyjevim testom za nezavisne uzorke i Wilcoxovim testom za zavisne uzorke. Rezultati: Ukupne kolorimetrijske vrijednosti pastila obrađenih samo gelom bile su 3024,42, onih tretiranih izvorom svjetlosti ZOOM 2 iznosile su 2999,74, pastila tretiranih Beyondom 2944,12 te laserom 2687,22. Najbolji rezultati postignuti su 30-postotnim gelom karbamid-peroksida i ZOOM-om 2, a slijede Beyond i femtosekundni laser, kod kojih je učinak izbjeljivanja bio proporcionalan trajanju tretmana i koncentraciji gela. Zaključak: Iako su se sva četiri postupka izbjeljivanja pokazala uspješnima, konačni rezultat ovisi o trajanju tretmana te vrsti i koncentraciji primijenjenoga gela.Objective: To study the efficiency of four different tooth whitening procedures we examined 30% carbamide peroxide gel alone and compared it with two available in-office whitening methods (Discus Dental, Culver City, CA, USA and Beyond Technology Corp, Santa Clara, CA, USA) and a procedure involving a femtosecond laser which consists of a 6W green laser pump (Millennium, Spectra Physics) and a mode-locked laser (Tsunami, Spectra Physics) which have not been clinically tested so far. Material and Methods: 40 pastilles of hydroxylapatite were used and immersed in green tea for 8 hours. After drying, pastilles were randomly divided into 4 groups, each consisting of 10 pastilles and treated with one of the 4 bleaching procedures. The color of pastilles was determined by the colorimeter in the RGB index prior to immersion into the tea, after the immersion and after the whitening treatment. Non-parametric tests were used for the analysis of colorimetric sum values - Kruskal-Wallis and Mann-Whitney test for independent samples and Wilcoxon test for dependent samples. Results: The colorimetric sum value for the pastilles treated with the gel only was 3024.42, for the pastilles treated with ZOOM 2 it was 2999.74, for the pastilles treated with Beyond it was 2944.12 and for the laser-treated pastilles it was 2687.22. The best results were obtained using the 30% carbamide peroxide gel and ZOOM2, followed by Beyond and the femtosecond laser method, which exhibited a whitening effect proportional to treatment duration and gel concentration. Conclusion: Although all four bleaching methods proved effective, the final result of bleaching depends upon the duration of treatment and the type and concentration of the gel applied